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Elution profile of an ion-exchange chromatography on a Mono Q FPLC column (0.5 by 5 cm) of a stock solution of NCS with a gradient of 0 to 0.3 M NaCl (flow rate, 1 ml/min). Proteolytic activity was observed only in the fraction containing protein (+). The apparent specific activity of this fraction was 5% of the apparent specific activity before chromatography, as estimated from the time required to proteolyze 50% of histone H1.
