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. Author manuscript; available in PMC: 2023 Sep 16.
Published in final edited form as: ACS Chem Biol. 2022 Sep 7;17(9):2448–2460. doi: 10.1021/acschembio.2c00199

Figure 5.

Figure 5.

PS-based site-specific Nanogold labeling of 3′SL RNA and distance distribution measurement by XSI. (A) Schematic for covalent conjugation of monomaleimido Nanogold to rTPT3αS-modified RNA via the thiol−maleimide reaction. (B) I0-normalized scattering profiles of U67 singly Nanogold-labeled (yellow), U97 singly Nanogold-labeled (red), U67/U97 doubly Nanogold-labeled (blue), and unlabeled 3′SLs (black). (C) Scattering interference patterns of the two rTPT3αS-conjugated (blue) or rTPT3A-conjugated (gray) Nanogold labels in 3′SL RNAs. (D) Probability distribution of the center-to-center distance distributions between the two rTPT3αS-conjugated (blue) or rTPT3A-conjugated (gray) Nanogold labels in 3′SL RNAs. (E) Schematic for covalent conjugation of mono-sulfo-NHS-Nanogold to rTPT3A-modified RNA via the amine-N HS ester reaction.