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. 2023 Jun 1;32(6):e4646. doi: 10.1002/pro.4646

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© 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Sequence analysis and clustering of smPGTs. (a). Table of characterized PGTs and their annotated preferred substrate assigned based on mature glycoconjugate composition. (b). Cladogram created from a multiple sequence alignment of a nonredundant set of 4684 smPGT sequences seeded with characterized PGT sequences. The cladogram reveals clear clustering based on UDP‐sugar specificity. The UDP‐diNAcBac cluster is highlighted in purple. (c). Sequence logo created from an MSA of the UDP‐diNacBac PGT cluster. Aromatic box residues are starred in purple, the “GLLLP” motif is starred in blue, and the conserved “PRP” nucleotide‐binding motif is starred in black.