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. 2023 May 3;9(5):1056–1063. doi: 10.1021/acsinfecdis.2c00598

Figure 3.

Figure 3

CD4-PP induces wound closure and clears in vitro infection. (A) Uninfected keratinocytes stimulated with CD4-PP showed a decrease in Ki-67 on the mRNA level. (B) Representative confocal imaging of Ki-67 protein expression shows a decrease in nuclear Ki-67 in CD4-PP-treated keratinocytes, depicting Ki-67 (green) and nucleus (blue). (C) Densitometric analysis of nuclear Ki-67 staining in keratinocytes with or without CD4-PP treatment. (D) Representative confocal images of keratinocytes fixed immediately postscarring. (E,F) Representative confocal images of MRSA-infected keratinocytes with or without CD4-PP treatment 48 h after scarring, depicting actin (green) and nucleus (blue). (G) Wound area size of MRSA-infected keratinocytes with or without CD4-PP treatment 48 h postscarring, showing significantly decreased wound area. (H) Representative confocal images of keratinocytes infected with MRSA CCUG 31966 with or without CD4-PP treatment, depicting nucleus (blue), LC3B (red), and p62 (green). (I) Densitometric analysis of LC3B in MRSA-infected keratinocytes with or without CD4-PP treatment. All microscopy imaging and densitometry analysis were performed in three independent experiments, with each experiment consisting of 4–5 random view fields. The average integrated density of each cell per set was used for statistical analyses (**p < 0.01, ***p < 0.001, unpaired t test).