FIG. 1.

(A) Genetic organization of the yopNtyeAsycNyscXY region of plasmid pCD1 in Y. pestis KIM. The locations of in-frame deletions within yscX and yscY are shown (see Materials and Methods). Plasmids pYSCX1 and pYSCY1 were used in complementation experiments. (B) Immunoblot analysis of culture supernatant and cell pellet fractions from Y. pestis strains grown at 37°C in the presence (+) or absence (−) of calcium. Antisera specific for YopN and V antigen were used to detect these proteins in the supernatant (S) and cell pellet (P) fractions from Y. pestis KIM8-3002 (parent), a yscV deletion mutant (ΔyscV), the yscX deletion mutant (ΔyscX), and the yscY deletion mutant (ΔyscY). The yscV, yscX, and yscY deletion mutants were complemented (/c) with plasmids pYscV1, pYSCX1, and pYSCY1, respectively.