TABLE 1.
Yeast two-hybrid analysis of YscX and YscY interactions
| Plasmids useda
|
Colony lift assayb | β-Gal activityc | |
|---|---|---|---|
| Derivative of pGAD424 | Derivative of pGBT9 | ||
| pGAD424 | pGBT9 | − | <1.0 |
| pGAD-YopE | pGBT-SycE | + | 111.9 ± 3.9 |
| pGAD-YscX | pGBT-YopN | − | <1.0 |
| pGAD-YscX | pGBT-TyeA | − | <1.0 |
| pGAD-YscX | pGBT-SycN | − | <1.0 |
| pGAD-YscX | pGBT-YscB | − | <1.0 |
| pGAD-YscX | pGBT-YscX | − | <1.0 |
| pGAD-YscX | pGBT-YscY | +/− | 5.7 ± 0.8 |
| pGAD-YscY | pGBT-YopN | − | <1.0 |
| pGAD-YscY | pGBT-TyeA | − | <1.0 |
| pGAD-YscY | pGBT-SycN | − | <1.0 |
| pGAD-YscY | pGBT-YscB | − | <1.0 |
| pGAD-YscY | pGBT-YscY | − | <1.0 |
| pGAD-YscY | pGBT-YscX | + | 120.0 ± 3.8 |
a
The SFY596 reporter strain was transformed with derivatives of the indicated plasmids.
b
Plus and minus signs indicate the relative intensity of the blue color developed after exposure of permeabilized cells to X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) for 1 h.
c
Expression of the reporter lacZ gene was measured as described by Clontech laboratories. The given values are averages (± standard deviations) assayed in triplicate. β-Gal, β-galactosidase.