IL36A and IL36G mRNA expression by RISH in acral samples. (a) Quantification of IL36A RISH in normal acral skin (n = 9), HPE (n = 12), PP (n = 8), and MFPP (n = 8) following the scoring system in Table 3. (b) Representative IL36A RISH (red) in PP tissue. (c) Quantification of IL36G RISH in normal acral skin (n = 9), HPE (n = 12), PP (n = 8), and MFPP (n = 8) following the scoring system in Table 4. (d) Representative IL36G RISH (red) in PP tissue. Middle lines represent medians; upper and lower ends of the boxes represent IQRs; whiskers represent values within 1.5 × IQR. Each point represents an individual case. Bar = 200 μm. HPE, hyperkeratotic palmoplantar eczema; IQR, interquartile range; MFPP, mycosis fungoides palmaris et plantaris; NL, normal acral skin; PP, palmoplantar psoriasis; RISH, RNA in situ hybridization.