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Stimulation of NADPH oxidation and inhibition of H2 production from NADPH by polysulfide. (A) H-II. (B) H-I. The 2-ml reaction mixture contained 100 mM EPPS (pH 8.0), 0.4 mM NADPH, polysulfide as indicated, and 250 μg of H-II (A) or 78 μg of H-I (B). The rate of NADPH oxidation was measured spectrophotometrically over 2 min. The amount of H2 produced (expressed as a percentage) was determined after 10 min.
