FIG. 3.

fadD1::Tn5 reduces hilA and invasion gene expression in a fadR-independent manner. (A) Complementation of fadD1::Tn5 by pN300, a plasmid containing E. coli fadD. β-Galactosidase activity for each fusion is expressed as a percentage of its activity in a wild-type SL1344 background. Average percentages were calculated by using three or more values from at least two different experiments. Error bars represent the standard deviation of normalized values. (B) fadR101 allows expression of fadF103::MudJ but does not suppress the effect of fadD1::Tn5 on hilA080::Tn5lacZY expression. Reporter fusions and fadD1::Tn5 were P22 transduced into TR6583 and LS1860. β-Galactosidase assays were performed on cultures grown in LB medium under oxygen-limiting conditions. β-Galactosidase activity is expressed as Miller units. Averages were calculated by using four or more values from at least two different experiments. Error bars represent the standard deviations. ND, not determined.