Table 2.

The top 20 significantly upregulated DE genes between the TB model and the normal group and their changes in various ag85a/b DNA versus TB model groups.

Genbank accession	Gene symbol	Fold Change values of the DE genes									Annotation
		TB model group versus Normal group	DNA IM group versus TB model group				DNA EP group versus TB model group
			10 μg	50 μg	100 μg	200 μg	10 μg	50 μg	100 μg	200 μg
NM_010491	Iapp	832↑	NO	NO	620↓	981↓	665↓	640↓	760↓	843↓	Islet amyloid polypeptide, has direct toxicity to islet B cells, mediates local inflammatory reaction, leading to pancreatic islet dysfunction, 39 , 40 , 41 an important pathological factor causing type 2 diabetes mellitus.
NM_009129	Scg2	428↑	NO	NO	391↓	363↓	382↓	345↓	396↓	399↓	Secretogranin II, has anti‐inflammatory properties and participates in inflammatory reaction, involves in the pathogenesis of diabetes. 42 , 43
NM_001042711	Amy2a5	365↑	NO	NO	239↓	356↓	285↓	272↓	457↓	400↓	Amylase alpha 2A, a member of the amylase family, involves in starch digestion and glycogen metabolism. 44
NM_001003405	Try5	337↑	NO	NO	314↓	295↓	330↓	287↓	313↓	239↓	Trypsin 5, involves in the hydrolysis of proteins and peptide chains.
NM_007693	Chga	326↑	NO	NO	433↓	440↓	317↓	342↓	440↓	408↓	Chromogranin A, has anti‐inflammatory properties and participates in inflammatory reaction, involves in the pathogenesis of diabetes. 43 , 45
NM_025350	Cpa1	309↑	NO	NO	312↓	297↓	274↓	305↓	307↓	307↓	Carboxypeptidase A1, hydrolyzes C‐terminal aromatic or neutral aliphatic amino acids residues of in proteins and polypeptide substrates.
NM_008100	Gcg	260↑	NO	NO	326↓	324↓	323↓	212↓	341↓	275↓	Glucagon, a kind of pleiotropic hormone with metabolic effects secreted by islet α cells, which can promote glycogen decomposition and gluconeogenesis to increase blood sugar, and also can promote fat decomposition and lipid oxidation. 46
NM_001126318	Gm13011	218↑	NO	NO	218↓	234↓	228↓	178↓	219↓	231↓	Elastase 3A, a member of the elastase family, involves in the hydrolysis of various proteins.
NM_198627	Vstm2l	198↑	NO	NO	199↓	136↓	180↓	182↓	197↓	197↓	V‐set and transmembrane domain containing two like, a novel modulator of neuroprotective activity. Overexpression of VSTM2L in a variety of cancer samples regulates IL‐4 signaling pathway, mainly enrichs in cell signal transduction, immune response, inflammatory response, calcium binding, and so forth. 47
NM_008386	Ins1	177↑	NO	NO	181↓	259↓	207↓	184↓	253↓	182↓	Insulin 1, a protein hormone secreted by the islets β cells, which can reduce blood sugar and promote the synthesis of glycogen, fat and protein.
NM_153518	Ccdc65	163↑	NO	NO	87↓	137↓	168↓	154↓	115↓	159↓	Coiled‐coil domain containing 65, a member of the coiled‐coil domain‐containing protein family, encodes many proteins like motor and skeletal proteins and involved in protein refolding and molecular recognition systems. 48
NM_011646	Try4	140↑	NO	NO	250↓	253↓	252↓	240↓	205↓	248↓	Trypsin 4, involved in the hydrolysis of proteins and peptide chains.
NM_009430	Prss2	135↑	NO	NO	179↓	206↓	151↓	198↓	205↓	137↓	Serine protease 2, a member of the trypsin family, plays a role in food digestion.
NM_029706	Cpb1	121↑	NO	NO	125↓	103↓	124↓	124↓	124↓	124↓	Carboxypeptidase B1, cleaves the C‐terminal of lysine or arginine, used as a serological marker of acute pancreatitis.
NM_026925	Pnlip	112↑	NO	NO	60↓	71↓	219↓	111↓	187↓	145↓	Pancreatic lipase, a member of the lipase family, secreted by the pancreas, hydrolyzes dietary triglycerides in the small intestine. 49
NM_007919	Cela2a	99↑	NO	NO	103↓	103↓	103↓	84↓	102↓	81↓	Chymotrypsin‐like elastase 2A, a member of the elastase family, circulates in plasma, reduces platelet hyperactivation, triggers insulin secretion and degradation, and increases insulin sensitivity. 50
NM_007694	Chgb	91↑	NO	NO	91↓	89↓	66↓	49↓	54↓	64↓	Chromogranin B, a member of the chromogranins/secretogranins protein family, has both extracellular and intracellular roles in the neuroendocrine system.
NM_009049	Resp18	81↑	NO	NO	80↓	81↓	76↓	63↓	43↓	59↓	Regulated endocrine specific protein 18, a novel secreted peptide, specifically expressed in mammalian central nervous system and endocrine system. 51
NM_172816	Slc30a8	78↑	NO	NO	58↓	44↓	72↓	80↓	69↓	60↓	Solute carrier family 30 member 8, a zinc transporter, transports Zinc from cytoplasm to insulin secretory granules in the pancreatic beta‐cells. 52
NM_007446	Amy1	75↑	NO	NO	53↓	57↓	62↓	65↓	54↓	58↓	Amylase 1, hydrolyzes 1,4‐alpha glycosidic bonds within starch, and plays a key role in starch digestion.

Abbreviations: DE, differentially expressed; EP, electroporation; IM, intramuscular injection; TB, tuberculosis; ↑, upregulated expression; ↓, downregulated expression.