Table 5.
The top 20 significantly downregulated pathway in TB model group and their changes in various ag85a/b DNA vaccine groups.
| Pathway ID | Definition | Enrichment score of the pathway | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TB model group versus Normal group | DNA IM group versus TB model group | DNA EP group versus TB model group | ||||||||
| 10 μg | 50 μg | 100 μg | 200 μg | 10 μg | 50 μg | 100 μg | 200 μg | |||
| mmu04060 | Cytokine‐cytokine receptor interaction | 6.389233↓ | NO | 1.617686↑ | 1.422668↑ | 5.262387↑ | 4.452331↑ | 2.437235↑ | 5.027105↑ | 3.917855↑ |
| mmu05133 | Pertussis | 4.154819↓ | NO | NO | 2.424137↑ | 3.131711↑ | 2.76652↑ | 2.846166↑ | 1.682164↑ | 2.482508↑ |
| mmu04974 | Protein digestion and absorption | 3.682461↓ | NO | NO | 6.066787↑ | 4.744358↑ | 2.866031↑ | 5.672109↑ | 7.386822↑ | 4.903948↑ |
| mmu04360 | Axon guidance | 2.756632↓ | NO | NO | 2.442363↑ | 4.352212↑ | 3.643141↑ | 3.325582↑ | 6.778728↑ | 3.843059↑ |
| mmu04933 | AGE‐RAGE signaling pathway in diabetic complications | 2.609451↓ | NO | NO | 4.077841↑ | 5.564438↑ | 3.175328↑ | 4.263249↑ | 5.156242↑ | 4.17245↑ |
| mmu04512 | ECM‐receptor interaction | 2.574761↓ | NO | NO | 8.116145↑ | 5.285638↑ | 3.243851↑ | 7.645445↑ | 11.261432↑ | 6.069831↑ |
| mmu04610 | Complement and coagulation cascades | 2.391915↓ | 1.769156↑ | NO | 5.643564↑ | 4.968025↑ | 4.035406↑ | 4.144754↑ | 5.654917↑ | 3.91844↑ |
| mmu00590 | Arachidonic acid metabolism | 2.263825↓ | NO | 2.628508↑ | 2.655805↑ | NO | NO | NO | NO | NO |
| mmu05150 | Staphylococcus aureus infection | 2.220857↓ | NO | NO | 2.522364↑ | 2.986781↑ | 2.540976↑ | 2.605515↑ | 3.381485↑ | 2.573856↑ |
| mmu00980 | Metabolism of xenobiotics by cytochrome P450 | 2.042694↓ | 2.112486↑ | 2.112486↑ | 2.886266↑ | 4.337605↑ | 1.437203↑ | 1.8995↑ | 3.070669↑ | 1.482553↑ |
| mmu00982 | Drug metabolism ‐ cytochrome P450 | 1.951735↓ | 2.076184↑ | 2.076184↑ | 3.331959↑ | 6.833289↑ | 1.754782↑ | 2.742079↑ | 5.387121↑ | 2.315482↑ |
| mmu05146 | Amoebiasis | 1.914775↓ | NO | NO | 2.30181↑ | 3.510302↑ | 2.393835↑ | 2.893891↑ | 4.645635↑ | 2.799971↑ |
| mmu04510 | Focal adhesion | 1.881851↓ | NO | NO | 6.102566↑ | 7.401803↑ | 4.954266↑ | 8.554973↑ | 9.255016↑ | 8.402425↑ |
| mmu04062 | Chemokine signaling pathway | 1.74527↓ | NO | NO | NO | 1.560615↑ | 1.451812↑ | 1.793169↑ | NO | NO |
| mmu00910 | Nitrogen metabolism | 1.662142↓ | NO | NO | 1.815201↑ | NO | NO | NO | 1.670625↑ | 1.841053↑ |
| mmu04350 | TGF‐beta signaling pathway | 1.656492↓ | NO | NO | 1.568223↑ | 2.109613↑ | NO | 2.298151↑ | 2.973736↑ | 2.02199↑ |
| mmu00730 | Thiamine metabolism | 1.629587↓ | NO | NO | NO | NO | NO | NO | NO | NO |
| mmu00480 | Glutathione metabolism | 1.550779↓ | NO | 2.292867↑ | 2.299118↑ | 2.709633↑ | NO | NO | 3.092353↑ | 1.838204↑ |
| mmu04151 | PI3K‐Akt signaling pathway | 1.526425↓ | NO | NO | 4.191528↑ | 3.487234↑ | 2.67112↑ | 4.990313↑ | 6.414413↑ | 5.464275↑ |
| mmu05144 | Malaria | 1.411386↓ | 1.42469↑ | 1.42469↑ | NO | 1.632998↑ | 1.708676↑ | 1.313239↑ | NO | NO |
Abbreviations: DE, differentially expressed; EP, electroporation; IM, intramuscular injection; TB, tuberculosis; ↑, upregulated score; ↓, downregulated score.