TABLE 1.

Diagnostic results and methodological aspects for all included studies in the analysis of synucleinopathies versus non‐synucleinopathies

Author	TP	FN	FP	TN	Sens	Spec	Assay	Cut‐off value
Groveman et al. 2018	27	2	0	31	0.93	1.00	RT‐QuIC	Mean of all samples +3SD
Manne et al. 2019	15	0	2	14	1.00	0.88	RT‐QuIC	Mean of all samples +10SD
Garrido et al. 2019	18	23	2	8	0.44	0.80	RT‐QuIC	Mean of negative controls +2SD
Van Rumund et al. 2019	62	21	5	72	0.75	0.94	RT‐QuIC	Mean of negative controls +2SD
Bongianni et al. 2019	43	11	2	57	0.80	0.97	RT‐QuIC	Mean of all samples +3SD
Rossi et al. 2020	166	39	10	224	0.81	0.96	RT‐QuIC	Mean of neuropathological controls +30SD
Orru et al. 2020	105	3	11	74	0.97	0.87	RT‐QuIC	10% of maximum value
Donadio et al. 2021	6	2	0	26	0.75	1.00	RT‐QuIC	Mean of negative controls +3SD
Iranzo et al. 2021	47	5	4	47	0.90	0.92	RT‐QuIC	Mean of negative controls +2SD
Bargar et al. 2021	143	3	0	68	0.98	1.00	RT‐QuIC	Mean background fluorescence +5SD
Rossi et al. 2021	77	4	20	188	0.95	0.90	RT‐QuIC	Mean of negative controls +30SD
Russo et al. 2021	24	4	1	29	0.86	0.97	RT‐QuIC	10% of maximum value
Brockmann et al. 2021	244	54	2	24	0.82	0.92	RT‐QuIC	Mean of negative controls +30SD
Poggiolini et al. 2021	113	30	2	53	0.79	0.96	RT‐QuIC	Mean of initial fluorescence at 120 h + 5SD
Mammana et al. 2021	22	1	0	57	0.96	1.00	RT‐QuIC	15% of maximum value
Perra et al. 2021	16	0	3	29	1.00	0.91	RT‐QuIC	Mean fluorescence during initial 10 h + 10SD
Sokratian et al. 2021	27	4	0	14	0.87	1.00	qRT‐QuIC	>200 FFUs/ml
Compta et al. 2022	19	38	4	51	0.33	0.93	RT‐QuIC	Mean of negative controls +2SD
Hall et al. 2022	68	13	13	49	0.84	0.79	RT‐QuIC	10% of maximum value
Shahnawaz et al. 2017	85	11	12	85	0.89	0.88	PMCA	≥50 FU
Singer et al. 2020	87	4	0	29	0.96	1.00	PMCA	≥150 AU
Shahnawaz et al. 2020	153	16	0	56	0.91	1.00	PMCA	≥50 FU

Abbreviations: SD, standard deviation; WT, wild type; FFUs, fibril forming units; FU, fluorescence units; AU, arbitrary units.