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. 2023 May 15;38(1):2205042. doi: 10.1080/14756366.2023.2205042

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 6. — The potent broad-spectrum anticancer hit compound P25 significantly inhibits colony formation capacity in A431 (A) and SK-MEL-28 (B) cells after 14 days. The percentage decrease in colony formation was concentration-dependent (0, ½IC₅₀, IC₅₀, and 1½IC₅₀ obtained from the antiproliferation assay) and was comparable in A431 (C, D) and SK-MEL-28 (E, F) cells. The data expressed in the bar graphs represent the mean ± SD in the P25-treated group expressed as a percentage relative to the untreated control group. Data are from three independent experiments performed in quadruplicate. Statistical significance was assessed using one-way ANOVA and Tukey’s multiple comparison tests; **p < 0.01, and ***p < 0.001 were considered significant.