Figure 8.

Compound P25 induces apoptosis by activating the extrinsic and intrinsic apoptotic pathways in cutaneous melanoma (SK-Mel-28) and non-melanoma (A431) cancer cells in culture. The blots (left) document a concentration-dependent effect (0, ½IC₅₀, IC₅₀, and 1½IC₅₀; IC₅₀ refers to the cell viability result, cf. Table 2) of protein expression levels of markers of apoptosis, including pro- and cleaved caspase-3, and caspase-9, and of cleaved PARP, after 48 h of treatment. The data shown are representative immunoblots from three independent experiments with similar results. β-actin was used as a loading control. At right, protein levels as normalised (mean ± SD of relative quantitative density values) are expressed as a percent increase or decrease vs. untreated cells (control) in the bar graphs. Statistical significance was assessed using one-way ANOVA and Bonferroni’s multiple comparison tests; *p < 0.05 and **p < 0.01, were considered significant.