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. 2023 May 11;186(10):2219–2237.e29. doi: 10.1016/j.cell.2023.04.003

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S2 — Overall, 3D and local resolution estimations, related to Figure 4

(A) Gold standard FSC (Fourier Shell Correlation) plots for the CryoSPARC reconstruction. Resolution was estimated at FSC = 0.143.

(B) Gold-standard (blue) and map vs. model (red) FSC plots. Resolution of gold-standard estimated at FSC = 0.143, model-vs-map estimated at FSC = 0.5.

(C) Directional FSC plots and sphericity values for the CryoSPARC reconstruction. These were calculated using a 3D-FSC server (https://3dfsc.salk.edu/).

(D) Gold standard (blue) and model-vs-map (red) FSC plots for the RELION4.0 reconstruction. Gold standard resolution was estimated at FSC = 0.143, model-vs-map resolution was estimated at FSC = 0.5.

(E) Directional FSC plots and sphericity values for the RELION4.0 reconstruction. These were calculated using a 3D-FSC server (https://3dfsc.salk.edu/).

(F) Local resolution estimates for the RELION4.0 reconstruction. The reconstruction was colored according to local resolution estimation in RELION.