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. 2023 May 11;186(10):2219–2237.e29. doi: 10.1016/j.cell.2023.04.003

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S6 — AlphaFold2 modeling of the DENND10-CCDC22-CCDC93 complex, related to Figure 5

(A) AlphaFold2 of human DENND10 and the CC1-CC2 coiled-coil domains of CCDC22 and CCDC93 colored by the confidence metric (pLDDT). The PAE plots of the top 2 ranked models are shown.

(B) Same model as in (A) but with DENND10 shown in surface representation colored according to conservation with CONSURF.¹¹³

(C) Top panel shows analytical size exclusion chromatography of DENND10 (magenta), CC1-CC2 complex (cyan) and DENND10 mixed with the CCDC22-CCDC93 forming a stable complex (orange). Bottom panel shows Coomassie stained gel of the peak fractions.

(D) Predicted DENND10 structure bound to CCDC22-CCDC93 in the same orientation alongside the crystal structure of DENND1B in complex with RAB35 (PDB ID: 3TW8).⁷⁰.