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Primer extension analysis of hprK transcription. RNA was prepared from S. xylosus C2a grown without and with glucose. Primer extension products were separated along with DNA sequencing reactions on an 8% polyacrylamide-urea gel. Lane 1, primer extension reaction with 15 μg of RNA from cells grown without glucose; lane 2, primer extension reaction with 15 μg of RNA from cells grown with 25 mM glucose.
