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[Preprint]. 2023 May 5:2023.05.05.539454. [Version 1] doi: 10.1101/2023.05.05.539454

Figure 1. Establishing SG landmarks.

Figure 1.

A. Co-localization of K5 (green) with K14 (red) in peripheral SG basal cells, but not in sebocytes. Middle and right panels are magnified single-channel views. B. Lack of co-localization of K5 with K79 (red) in sebocytes. C. Co-localization of K5 with PPARγ (red) in transitional basal cells of the lower SG (arrows). D. Co-localization of PPARγ (red) with Ki67 (green) in a subset of peripheral basal cells (arrows) in the SG. E. Schematic of telogen hair follicle. Note that the infundibulum and sebaceous ducts are continuously lined by differentiated K79+ cells (red). F. Localization of K79 (green) and PPARγ (red) in the developing hair follicle during embryonic (E) days 16.5–17.5. Middle panels, follicle with basal PPARγ+ cells (arrowhead), but minimal co-localization with K79. Right panels, follicle with early sebocytes identified by the unique co-localization of PPARγ and K79 (arrow). Dotted lines delineate the basal layer of the epidermis and hair follicle. Bottom panels are magnified views, with DAPI omitted for clarity. G. Schematic of SG specification. PPARγ+ basal cells (orange) initially emerge at E16.5–17.5 and give rise to early sebocytes (pink) adjacent to the K79+ cell column (red). Subsequent remodeling leads to the opening of the sebaceous duct and hair canal (Mesler et al., 2020). One of two SG lobes is depicted. The second lobe may be specified later, or may arise when the initial SG compartment splits into two, as has been proposed (Frances and Niemann, 2012).