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[Preprint]. 2023 May 3:rs.3.rs-2852140. [Version 1] doi: 10.21203/rs.3.rs-2852140/v1

Figure 2. Technical validation of RNA-seq data using qPCR experiments.

Figure 2

Scatterplots of qPCR assays (ddCt-RPL13A, × axis) of 9 genes plotted against the results of RNA-seq (RNAseq-log2FC, Y axis), where RNAseq-log2FC is the difference between the log2 normalized expression for each sample compared to the average log2 normalized expression for healthy control samples. The measurements with both gene expression quantification platforms were in excellent agreement for all phenotypes.