TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| H. influenzae | ||
| KW20 | Wild type | H. O. Smith |
| MAP7 | str kan nov nal spc vio stv | J. Setlow; 17 |
| RR514 | str | 32 |
| RR520 | kan | 32 |
| RR563 | sxy-1 | 32 |
| RR749 | murE749; G1303→A | This study |
| RR750 | murE750; G1303→A | This study |
| RR751 | murE751; T1082→C | This study |
| RR752 | murE752; G1303→T | This study |
| RR769 | rec-2::mini-Tn10kan murE749 | This study |
| RR797 | Cmr cassette from pKRP10 inserted into HindIII site at bp 1195829 in strain RR749 | This study |
| RR804 | murE749 transformed into RR514 background | This study |
| RR806 | murE750 + CAT | This study |
| RR807 | murE751 + CAT | This study |
| RR808 | murE752 + CAT | This study |
| RR809 | murE+ + CAT | This study |
| RR829 | crp::mini-Tn10kan murE749 | This study |
| RR830 | icc::spec murE749 | This study |
| RR839 | sxy::lacZ fusion | 1a |
| RR867 | Insertion and duplication of comA; comA::lacZ Cmr | 19 |
| RR868 | rec2::lacZ fusion | 19 |
| RR876 | sxy::lacZ fusion murE749 | This work |
| RR878 | comA::lacZ fusion murE749 | This work |
| RR879 | rec2::lacZ fusion murE749 | This work |
| E. coli | ||
| DH5α | sup44 recA1 | |
| GM2163 | dam | New England Biolabs |
| Plasmids | ||
| pRRnov1 | H. influenzae n-vobiocin resistance allele of gyrA | 32 |
| pWJC3 | Kmr cassette | 9 |
| p836B | murE749KpnI-BglII fragment from RR797 | This work |
| p848M | murE+ KpnI-BglII fragment from RR805 in pSU40 | This work |
| p836B-20 | 6,845-bp HindIII fragment from p836B subcloned into pSU20 | This work |
| p848M-20 | 6,845-bp HindIII fragment from p848M subcloned into pSU20 | This work |
| pmurE::Kan | Kmr cassette from pWJC3 inserted into PstI site of p848M-20 | This work |
| pAM120 | Tn916 vector | 14 |
| pKRP10 | Cmr cassette | 33 |