FIGURE 2.

Translation reinitiation is more efficient after small uORFs in vitro and in HeLa cells. (A,B) Response of nLuc reporters that harbor a small, mutant, large, or truncated uORF with a 16 nt or 115 nt intercistronic spacer from (A) in vitro translation and (B) in HeLa cells. n = 3 biological replicates. Bar represents the mean. Luminescence signals were set relative to the control no uORF reporter (in black). Comparisons against the control (in black) and experimental reporters (in red) were made using a two-tailed unpaired t-test with Welch's correction. (*) P < 0.05. (**) P < 0.01. (***) P < 0.001. (****) P < 0.0001. Exact P-values can be found in Supplemental Table 2.