Figure 7.

Siponimod treatment inhibited lymphocyte infiltration and alleviated Th cell infiltration and activation in the ICH brain on day 3 after ictus (Flow cytometry analysis). (A) Gating strategies for CD45⁺, CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD3^-NKp46⁺, CD3^-CD19⁺, and CD3⁺CD4⁺CD69⁺ cells. (B) Representative flow cytometry plot for CD45⁺, CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD3^-NKp46⁺, CD3^-CD19⁺, and CD3⁺CD4⁺CD69⁺ cells in brain samples from vehicle- and siponimod-treated mice on day 3 after ICH. (C) Percentages of lymphocytes, Th cells, CTLs, NK cells, and B lymphocytes and the MFI of CD69 in Th cells in vehicle- and siponimod-treated mice on day 3 after ICH (t-test, n = 6 mice per group). All data are expressed as mean ± SD.