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. 2023 May 2;11:1182404. doi: 10.3389/fchem.2023.1182404

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Copyright © 2023 Lim, Yagnik, Henkel, Frost, Bien and Rothschild.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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4-Plex MALDI-IHC image of FF normal rat kidney tissue section. (Upper Left) 18 mm² (5.08 × 3.52 mm) area of the kidney tissue which consists of 715,264 individual pixels which took a total of approximately 11 h of data acquisition (∼18 pixels/s) on a timsTOF fleX equipped with microGRID and recorded at 5 µm pixel size. (Lower Right) 5x magnification of the area shown in the hatched green box which is a portion of the renal cortex. The yellow bracket indicates the general vicinity of the kidney medulla. Base colors for both images are Na⁺/K⁺ ATPase (purple), vimentin (red), collagen (green), and panCK (blue).