TABLE 1.
Comparison of the major features of various multiplex tissue imaging methods with focus on top-down targeted imaging of intact proteins. “*” denotes imaging methods which are intrinsically bottom-up and do not target specific intact proteins. For example, MALDI-MSI is normally used for untargeted detection of small molecules including protein proteolytic fragments. Note that the last row with yellow check marks combines features of MALDI-MSI and MALDI-IHC.
| Target plex limit | Unlabeled small molecules | Intact targeted proteins | Cellular resolution | Quickly scans entire slide | |
|---|---|---|---|---|---|
| H&E | * |
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| IF | 10 |
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| MIBI | 45 |
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| IMC | 45 |
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| CyclicIF | 100 |
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| MALDI-MSI | * |
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| MALDI-IHC | >100 |
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| Combined MALDI-IHC Workflow (High-plex, Multimodal, Multiomic) | >100 |
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