Extended Data Fig. 2. Generation of mice with deletion of the DNS enhancer and interaction of the DNS enhancer with the canonical Notch1 and DNS promoters.

(a) Strategy to generate the DNS enhancer mutant allele. A 600 bp region containing the conserved TF BS were deleted by CRISPR-Cas9-mediated gene editing. DNS1 (red square) and DNS2 and 3 (blue square), and TF BS (yellow square) are shown by respectively colored squares. (b) Statistically significant (FDR <0.01) long distance interactions at Notch1 locus identified by HiC in DN2 thymocytes are shown together with histone modifications and chromatin accessibility.

HiC data shown in (b) was downloaded publicly available data (GSE90958).