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. 2023 May 16;12:e86413. doi: 10.7554/eLife.86413

Figure 3. Lack of dietary aryl hydrocarbon receptor (AhR) ligands worsens airway allergy after skin sensitization.

Figure 3.

Mice were placed on AhR-poor or indole-3-carbinol (I3C) diet for 3 wk of adaptation prior to the start of experiments. Papain or vehicle (PBS) was injected in the footpad at day 0. At day 7, all mice were placed on the I3C diet. Mice were exposed to papain intranasally four times at days 14, 15, 27, and 35. 24 hr after the last exposure, bronchoalveolar lavage (BAL) and lung tissue were analyzed. (A) Cell counts in BAL (n = 12 in three independent experiments). (B) Cytokine concentration in BAL (n = 12 in three independent experiments). (C) Lung lysates were analyzed by RT-qPCR (n=18 in four independent experiments). For all panels, median is shown. Kruskal–Wallis test. *p<0.05; **p<0.01.