Strain, strain background (mouse) |
Cd207(Langerin)-eGFP-DTR |
Kissenpfennig et al., 2005
|
RRID:IMSR_JAX:016940
|
Founder animals were donated by M.Vocanson (CIRI, Lyon, France) |
Cell line (human) |
HaCaT |
Wilson, 2014
|
RRID:CVCL_0038
|
|
Antibody |
Anti-TCRβ BUV737 (hamster monoclonal, clone H57-597) |
BD Biosciences |
AB_2740818 |
1:200 |
Antibody |
Anti-CD11c PerCpCy5.5 (hamster monoclonal, clone HL3) |
BD Biosciences |
AB_1727422 |
1:200 |
Antibody |
Anti-SiglecF BV480 (rat monoclonal, clone E50-2440) |
BD Biosciences |
AB_2743940 |
1:200 |
Antibody |
Anti-CD45 FITC (rat monoclonal, clone 30-F11) |
BioLegend |
AB_312973 |
1:200 |
Antibody |
Anti-CD11b Pe-CF594 (rat monoclonal, clone M1/70) |
BD Biosciences |
AB_11154422 |
1:400 |
Antibody |
Anti-MHCII BV786 (rat monoclonal, clone M5/114.15.2) |
BioLegend |
AB_2565977 |
1:200 |
Antibody |
Anti-Ly6G BV605 (rat monoclonal, clone 1A8) |
BioLegend |
AB_2565880 |
1:200 |
Antibody |
Anti-CD4 BV650 (rat monoclonal, clone RM4-5) |
BioLegend |
AB_2562529 |
1:200 |
Antibody |
Anti-Ly6C AF700 (rat monoclonal, clone HK1.4) |
BioLegend |
AB_10643270 |
1:300 |
Antibody |
Anti-EpCAM APCFire750 (rat monoclonal, clone G8.8) |
BioLegend |
AB_2629758 |
1:400 |
Antibody |
Anti-CD11b PerCPCy5.5 (rat monoclonal, clone M1/70) |
BD Biosciences |
AB_394002 |
1:400 |
Antibody |
Anti-TCRβ APC (hamster monoclonal, clone H57-597) |
BioLegend |
AB_313435 |
1:200 |
Antibody |
Anti-CD4 FITC (rat monoclonal, clone RM4-5) |
BD Biosciences |
AB_394582 |
1:200 |
Antibody |
Anti-CD19 APC-Cy7 (rat monoclonal, clone 1D3) |
BD Biosciences |
AB_396770 |
1:200 |
Antibody |
Anti-CD172a BUV737 (rat monoclonal, clone P84) |
BD Biosciences |
AB_2871154 |
1:200 |
Antibody |
Anti-CD19 BV480 (rat monoclonal, clone 1D3) |
BD Biosciences |
AB_2739509 |
1:200 |
Antibody |
Anti-CD3 BV480 (hamster monoclonal, clone 500A2) |
BD Biosciences |
AB_2744035 |
1:200 |
Antibody |
Anti-XCR1 BV510 (rat monoclonal, clone ZET) |
BioLegend |
AB_2565231 |
1:100 |
Antibody |
Anti-CD11c BV785 (hamster monoclonal, clone N418) |
BioLegend |
AB_2565268 |
1:100 |
Antibody |
Anti-CD86 FITC (rat monoclonal, clone GL1) |
BD Biosciences |
AB_394993 |
1:100 |
Antibody |
Anti-CD26 PE (rat monoclonal, clone H194-112) |
BioLegend |
AB_2293047 |
1:200 |
Antibody |
Anti-CD40 PerCP-efluor710 (rat monoclonal, clone 1C10) |
eBioscience |
AB_2573677 |
1:100 |
Antibody |
Anti-PDL2 APC (rat monoclonal, clone TY25) |
BioLegend |
AB_2566345 |
1:100 |
Antibody |
Anti-MHC II BV650 (rat monoclonal, clone M5/114.15.2) |
BioLegend |
AB_2565975 |
1:400 |
Software, algorithm |
DESeq2
|
Love et al., 2014
|
v1.22.2 |
|
Software, algorithm |
GSEA |
Subramanian et al., 2005
|
v4.0.3 |
|
Software, algorithm |
QuPath
|
Bankhead et al., 2017
|
v.0.3.1 |
|
Software, algorithm |
FlowJo |
FlowJo LLC |
v10 |
|