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. 2023 Apr 14;15(8):3021–3034. doi: 10.18632/aging.204655

Figure 2.

Figure 2

miR-10a-5p was the target gene of TUSC7. (A) After transfection with TUSC7 overexpression plasmid and shRNA plasmid, q-PCR was used to measure transfection efficiency. (B) Expression of miR-10a-5p after overexpression and silencing of TUSC7 in U251 and U87 cells. (C) Binding site of miR-10a-5p in the TUSC7 transcript. (D) The results of dual-luciferase reporter gene assay showed that the relative luciferase activity was inhibited after co-transfection of wild-type (WT) plasmids and miR-10a-5p mimics, while it was not inhibited after transfection of mutant vector (MUT). **p<0.01; ns: p>0.05.