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. 2023 May 16;14:2804. doi: 10.1038/s41467-023-38482-w

Fig. 7. Cell morphology and cortical actin recruitment depend on Yap activity.

Fig. 7

A Confocal microscopy images of medial and lateral (DMSO) and lateral (Rockout-treated) converging cells from embryos injected with Utrophin::GFP, and LyndTm (B) and stained with DAPI. XZ projections from the sections indicated with white rectangles are shown (A’). Schematic representation of the embryo indicating the area of interest with a magenta rectangle is shown on the left side of the panel. Cell shapes are represented with white lines in the images corresponding to DAPI. B Quantification of average cell compactness in DMSO and Rockout-treated embryos. C Quantification of the average number of filopodia in DMSO and Rockout-treated embryos. D Quantification of average nuclei flatness in DMSO and Rockout-treated embryos. Detailed plot in supplementary figure 6. Boxes represent the quartiles; the whiskers indicate the maximum and minimum values. Red and black lines indicate the median and the mean, respectively. Points indicate independent embryos. n = 7 DMSO treated embryos examined in medial regions, n = 7 DMSO treated embryos examined in lateral regions, n = 8 Rockout-treated embryos. P values are indicated in the figure. Two-sided Student’s t tests were performed to evaluate statistical significance. Scale bars = 5 µm. Source data are provided as a Source Data file.