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. 2023 May 16;14:2804. doi: 10.1038/s41467-023-38482-w

Fig. 9. Yap senses intracellular tension within a mechanoregulatory feedback loop.

Fig. 9

A ISH analysis distribution of marcksl1b in control (untreated) and compressed WT embryos during 20 min. Lateral binocular images are shown. Confocal microscopy images of marcksl1b fluorescent ISH stained with DAPI from the sections indicated with yellow rectangles are shown. B Quantification of marcksl1b fluorescent ISH signal intensity in control and compressed embryos. P value = 0.021. Boxes represent the quartiles; the whiskers indicate the maximum and minimum values. Red and black lines indicate the median and the mean, respectively. Points indicate independent embryos. n = 7 control embryos, n = 6 compressed embryos. C mRNA levels of ctgfa, lats2, lamc1, and marcksl1b in control and compressed embryos as quantified by RT-qPCR. P values are indicated in the figure. Data are represented as mean ± SD; points indicate technical replicates. n = 20 embryos. D Summarizing scheme representing the differences between medial and lateral migrating cells converging to the midline in WT, yap1−/−;yap1b−/− and reduced tension embryos. E Main components of the Yap-dependent transcriptional program encode for proteins that provide a link between the ECM and the actin cytoskeleton. Two-sided Student’s t tests were performed to evaluate statistical significance. Scale bars are 100 µm and 10 µm (A). Source data are provided as a Source Data file.