Skip to main content
. 2023 May 16;14:2241. doi: 10.1038/s41467-023-37714-3

Fig. 3. N-Glycan biosynthesis is essential for AMA-induced cell death.

Fig. 3

a A simplified overview of N-glycan biosynthesis. b KIF blocked AMA toxicity. HAP1 cells were pre-treated with KIF for 12 h and then treated with AMA (3 μM) for 48 h (n = 3 biological replicates). c Both pre-treatment and post-treatment with KIF protect HAP1 cells against AMA (3 μM) (n = 6 biological replicates). nsp = 0.9117, ****p < 0.0001. The statistics were assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. d, e Depletion of STT3B conferring resistance to AMA in HAP1 d and HepG2 e cells (n = 6 biological replicates). ****p < 0.0001. The statistics were assessed using one-way ANOVA followed by Dunnett’s multiple comparisons test. f, g The detection of AMA entrance into cells. The representative peak and relative peak area (n = 5 biological replicates) of AMA in different sgRNA STT3B knockout HAP1 f, and HepG2 g cells. ***p = 0.0003, ***p = 0.0006; ***p = 0.0002, **p = 0.0010. The statistics were assessed using one-way ANOVA followed by Dunnett’s multiple comparisons test. h, i The expression of OATP1B3 and NTCP in different sgRNA STT3B knockout HAP1 h and HepG2 i cells. Data are presented as mean ± S.D.  and are representative of three independent experiments. Source data are provided as a Source Data file.