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. 2023 May 16;14:2241. doi: 10.1038/s41467-023-37714-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Intracellular co-localization of ICG and ER in HAP1 and HepG2 cells and the corresponding fluorescence intensity profiles across the cell along the direction of arrow. b A scheme of ER-LucT reporter system, the disruption of luciferase glycosylation turn on luminescence. Luc, luciferase. c N-glycosylation of Luc reduced the Luc activity in HAP1 cells (n = 3 biological replicates). **p = 0.0012 d The blockage of N-glycosylation of Luc increased the Luc activity by treatment with 10 μM ICG. The ER-LucT-activity was normalized to the vehicle control (n = 3 biological replicates). ****p < 0.0001. e N-glycosylation of Luc reduced the Luc activity in HAP1 cells (n = 3 biological replicates). ****p < 0.0001. f The blockage of N-glycosylation of Luc increased the Luc activity by treatment with 100 μM ICG HepG2. The ER-LucT-activity was normalized to the vehicle control (n = 3 biological replicates). ***p = 0.0001. Data are presented as mean ± SD and are representative of three independent experiments. The statistics were all assessed using two-tailed unpaired t test. Source data are provided as a Source Data file.