Figure 4.

Single-cell RNA sequencing data analysis of LYVE⁺ tumorigenic TAMs cells identified the cellular trajectories responsible for tumor growth

(A) Schematic diagram of sample collection and single-cell analysis workflow. (B) Uniform Manifold Approximation and Projection (UMAP) of the filtered data (23,250 cells) clustered into 14 clusters. Each cell is represented as a dot. (C) Schematic representation of the differential expression analysis performed between all EV_EOMA treated versus all untreated Mφ and between cluster 7 versus cluster 4. (D) Trajectory inference of cells from clusters 4 and 7 colored by clusters (left), pseudotime (middle), or the originating sample (right). (E) Violin plots representing expression level of Ccl2 in Mφ, Mφ_{miR126 null EV}, and in Mφ_{EOMA EV} cells (upper). Bar plots of PCR results for Ccl2 (below). (F) Network of upregulated genes found in both comparisons Mφ _{EOMA EV} versus Mφ or in cluster 7 versus cluster 4 cells. Nodes with red colors represent genes found to be upregulated in both comparisons Mφ_{EOMA EV} versus mφ and cluster 7 cells versus cluster 4 cells. Nodes with blue color represent genes found to be upregulated in Mφ_{EOMA EV} versus Mφ cells, but not in cluster 7 cells versus cluster 4 cells. Nodes with golden color represent genes found to be upregulated in cluster 7 cells versus cluster 4 cells, but not in Mφ_{EOMA EV} versus Mφ cells. Data presented as mean ± SEM.