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. 2023 Feb 18;31(5):1468–1479. doi: 10.1016/j.ymthe.2023.02.016

Figure 1.

Figure 1

Isolation and quantification of mitochondria using flow cytometry

(A) Immunoelectron microscopy and immunofluorescence images of isolated mitochondria. (B) Mitochondrial amount (Mitotracker green), mitochondrial membrane potential (TMRM) and mitochondrial superoxide levels (MitoSox) in cells and isolated mitochondria were evaluated using flow cytometry (n = 3 independent cultures; 30,000–100,000 events per run). Data are shown as mean ± standard error of the mean. Significance was determined by one-way ANOVA for the remaining panels. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.