Figure 7.

NTB-DCs vaccine significantly reduce the abundance of F. nucleatum in colon, numbers of CRC tumors and improved the survival rate of CRC mice. (A) PET/MR merge images of mice. (B) Comparison of ¹⁸F-FDG uptake rates in the ROI of mice colon after PBS and NTB-DCs treatment. (%ID/g: % injected dose per gram of ¹⁸F-FDG in the ROI.) (C) Intestinal tissue from CRC mice colorectum were collected and the representative histopathological sections are shown (magnification: x100). Severity scores of intestinal tissues were shown in the figure. (D) Diluted cell lysates from CRC mice colon tissues were plated on Anaerobe Basal Broth plates and colonies were counted after 48h. The F. nucleatum burdens in were measured. (E) The numbers of CRC from mice colorectum were counted after sacrifice. Mice are grouped according to the number of tumors and presented in the figure as a percentage. (F) The survival rates were monitored from 18 to 24 week (n=10 per group by log-rank [Mantel-Cox] test). The results are shown as the means ± SD. *P <0.05; **P < 0.01; ***P < 0.001.