Table 1.
Immune mass cytometry metal-labeled antibodies.
| N | Marker | Metal | Description | Clone | Vendor | Titration (1:X) | Cat# |
|---|---|---|---|---|---|---|---|
| 1 | CD45RO | 139La | Memory T-cells | T200/797 | Abcam | 500 | ab212786 |
| 2 | CCR6 | 141Pr | Treg | EPR22259 | Abcam | 1000 | ab243852 |
| 3 | CD137 | 142Nd | Check point | BLR051F | Bethyl | 25 | A700-051. |
| 4 | ICOS | 143Nd | Check point | D1K2T | CST | 50 | 89601BF |
| 5 | PD-L1 | 144Nd(2X) | Check point | E1L3N | CST | 50 | 13684BF |
| 6 | CD68 | 145Nd | Macrophage | EPR20545 | Abcam | 1000 | ab227458 |
| 7 | MPO | 146Nd | Neutrophil | EPR20257 | Abcam | 2500 | ab221847 |
| 8 | PD-1 | 147Sm | Check point | EPR4877 | Abcam | 25 | ab186928 |
| 9 | CD11c | 148Nd | Dendritic cell | EP1347Y | Abcam | 2000 | ab216655 |
| 10 | CD73 | 149Sm | Check point | BLR054F | Bethyl | 500 | A700-54. |
| 11 | HLA-DR | 150Nd | Antigen Presentation | EPR3692 | Abcam | 350 | ab215985 |
| 12 | CD163 | 151Eu | Macrophage | BLR087G | Bethyl | 400 | A700-087. |
| 13 | Granzyme B | 152Sm | T-cell Activation | D6E9W | CST | 1000 | 46890BF |
| 14 | CD11b | 153Eu | Macrophage | EPR1344 | Abcam | 500 | ab216445 |
| 15 | CD14 | 154Sm | Macrophage | EPR3653 | Abcam | 1500 | ab214438 |
| 16 | FOXP3 | 155Gd | Treg | BLR034F | Bethyl | 25 | A700-034. |
| 17 | TIM3 | 156Gd | Checkpoint | D5D5R | CST | 200 | 45208BF |
| 18 | CD66b | 158Gd | Activated neutrophil | EPR7701 | Abcam | 100 | ab249555 |
| 19 | LAG3 | 159Tb | T-cell Activation | D2G4O | CST | 200 | 15372BF |
| 20 | CD39 | 160Gd | Treg | EPR20461 | Abcam | 250 | ab232573 |
| 21 | IDO-1 | 161Dy | Treg | D5J4E | CST | 25 | 86630BF |
| 22 | Ki-67 | 162Dy | Proliferation | D2H10 | CST | 200 | 9027BF |
| 23 | VISTA | 163Dy | Checkpoint | D1L2G | CST | 50 | 64953BF |
| 24 | β2-microglobulin | 164Dy | Other, MHC-I | D8P1H | CST | 200 | 12851BF |
| 25 | CD19 | 165Ho | B-cells | D4V4B | CST | 1500 | 90176BF |
| 26 | CD8a | 166Er | CD8 cells | D8A8Y | CST | 100 | 85336BF |
| 27 | CD33 | 167Er | Myeloid lineage | SP266 | Abcam | 600 | ab238784 |
| 28 | B7-H3 | 168Er | Check point | D9M2L | CST | 25 | 14058BF |
| 29 | CD45 | 169Tm | Lymphocytes | EP322Y | Abcam | 800 | ab214437 |
| 30 | CD94 | 170Er | NK cell/cytotoxic lymphocytes | EPR21003 | Abcam | 50 | ab238166 |
| 31 | OX40 | 171Yb | T-cell Activation | E9U7O | CST | 25 | 61637BF |
| 32 | CD3e | 172Yb | Lymphocytes | D7A6E | CST | 100 | 85061BF |
| 33 | CD4 | 173Yb | T-helper Lymphocyte | EPR6855 | Abcam | 1000 | ab181724 |
| 34 | Cytokeratin | 174Yb | Epithelial | AE1/AE3 | Abcam | 25 | ab80826 |
| 35 | GAPDH | 175Lu | Other, control | 14C10 | CST | 5000 | 2118BF |
| 36 | NaKATPase | 176Yb | Other, control | EP1845Y | Abcam | 200 | ab167390 |
Thirty-six validated markers designed to include lymphoid, myeloid, immune-modulatory, epithelial, proliferative, and leukocyte/constitutive.
'#' is a short-hand abbreviation for number.