Fig. 1.

HSF5 is a male germ cell-specific protein and high expression on XY body of pachytene spermatocytes. (A) qPCR analyses of Hsf5 mRNA expression in multiple tissues in male mice. (B) Expression of HSF5 protein in multiple tissues in male mice detected by Western blot. β-actin is serve as loading control. (C) Immunofluorescent staining of HSF5 and γH2AX on testis section. γH2AX was used as a marker for sex chromosomes in pachytene spermatocytes. Nuclei were stained with DAPI. The inset is an enlarged view of a different stage spermatocytes. Scale bar, 50 μm. (D) Immunostaining of HSF5 (red) and SYCP3 (green) on chromosome spreads of spermatocytes from wild type testes, the magnification box indicates XY body, Scale bars, 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)