Fig. 4.

Hsf5 deletion activates CHK2 checkpoint leading to germ cell apoptosis. (A) Expression of SMARCA4 protein in wild type and Hsf5^−/− spermatocyte detected by Western blot. Β-actin is served as loading control. (B) qPCR analyses of Smarca4 mRNA expression in wild type and Hsf5^−/− spermatocyte. (C) Testicular sections were stained for SMARCA4.The inset is an enlarged view of single spermatocytes. Scale bar, 50 μm. (D) Testicular sections were stained for H3K9me3.The inset is an enlarged view of single spermatocytes. Scale bar, 50 μm.