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. 2021 Apr 28;13(4):984–994. doi: 10.1177/21925682211012323

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Histological analysis and immunohistochemistry. A, HE staining showing the structural integrity of the intervertebral disc decreased with increasing ischemia. B, Alcian blue staining indicating that the content of proteoglycan decreases with increasing ischemia. C, Toluidine blue staining indicates that the calcified layer of the intervertebral disc thickens with increasing ischemia. D, With increasing degree of ischemia, the positive expression of collagen II decreased progressively. E, The expression of GAP43 in the intervertebral disc is enhanced with increasing ischemia. F, The expression of PGP9.5 is enhanced with increasing ischemia. The boxed regions are shown at 40 × magnification (scale bar = 100 μm).