Figure 1.

Schematic overview and naming convention of antibody-targeted proteases. A, the antibody-guided enzyme combines the favorable properties of antibodies and enzymes while mitigating the drawbacks of each. B, nontargeted and targeted formats for the antibody–enzyme fusion proteins used in this work. The naming convention is structured as follows. The first label refers to the molecule format (Fc: fragment crystallizable, Fab: fragment antigen binding, scIgG: monovalent single-chain Immunoglobulin G, IgG: immunoglobulin G). By definition, “Fab” is monovalent and “Fc” has no targeting arms. Enz refers to the free enzyme. The center label describes the fusion format as either NTF (N-terminal fusion) or CTF (C-terminal fusion). When necessary, further clarification of the fusion domain is specified within the parentheses. The third and final label denotes the number of proteases per molecule as either one or 2. LC, light chain.