FIGURE 7.

Mechanically induced osteogenesis is dependent on actomyosin contractility (a) mRNA expression levels of osteogenic markers in the static and dynamic conditions with/without the inhibitors of actomyosin contractility. (b) Immunofluorescence of type 1 collagen and (c) quantification of fluorescent intensity. (d) Alkaline phosphatase staining and (e) quantification of extracted substrate. (f) Alizarin red S staining and (g) quantification of extracted dye. The samples for the mineralization assay were cultured in the presence of β‐glycerophosphate as a source of phosphate ions. Statistical comparison between the static and dynamic condition was performed by the Student's t‐test and comparison among pharmacologically treated groups were by ANOVA followed by Dunnett's multiple comparison. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. White scale bar = 100 μm, black scale bar = 500 μm.