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. 2022 Dec 13;8(3):e10466. doi: 10.1002/btm2.10466

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© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of The American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) Evaluation of periodontal bone regeneration by Masson staining at 2 weeks after surgery. (b) Quantitative analysis of periodontal bone regeneration by Masson staining, including the width of the buccal new bone and new bone area fraction, at 2 weeks after surgery. (c) Evaluation of periodontal bone regeneration by Masson staining at 4 weeks after surgery. (d) Quantitative analysis of periodontal bone regeneration by Masson staining, including the width of the buccal new bone and new bone area fraction, at 4 weeks after surgery. NB, new bone; OB, original bone. (e) Immunohistochemical staining of osteocalcin (OCN) in the defect area at 2 weeks. (f) Quantitative evaluation of the integral optical density (IOD) of OCN. (n = 3) (*p < 0.05, **p < 0.01)