S100A9 expression increased in the aged aortas of mice and humans. (A) S100A9 expression in the aortas of mice at 3-, 12- and 24-month was measured using Western blots. The statistical results of S100A9 were presented in (B) (n = 6). (C) S100A9 expression in mice aortas was measured using IHC at 3- and 24-month and the related statistical results were displayed in (D) (n = 4). (E) S100A9 expression in young and aged human aortas was measured using Western blots. (F) The statistical results of blots in E (young group, <40 years, n = 3; aged group, >60 years, n = 5). (G) Representative IHC images of S100A9 expression in the aorta of young and aged humans and the related statistical results were depicted in (H) (n = 3). ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001.