Fig. 7.

S100A9 induces cellular senescence in human aortic endothelial cells via TLR4. (A) Representative Western blots of Nox1 in HAECs treated with rhS100A9 with TLR4 or RAGE inhibitors. The statistical results were presented in (B) (n = 6). (C–E) The results of NADPH oxidase and Sirtuin activities and NO content in HAECs from different groups (n = 6). (F–G) Representative pictures of SA-β-Gal-stained HAECs and quantification of SA-β-Gal-staining positive cells (n = 5). ns, not significant. *P < 0.05, **P < 0.001, ***P < 0.001, ****P < 0.0001.