Skip to main content
. 2023 May 3;25(6):289. doi: 10.3892/etm.2023.11988

Figure 2.

Figure 2

IL-37 inhibits ferroptosis of macrophages and oxidative stress caused by HG/ox-LDL. The macrophages were divided into three groups: i) CTRL group; ii) HG/ox-LDL group: The macrophages were treated with 100 ng/ml ox-LDL and 25 mM glucose for 24 h; and iii) IL-37 group: 30 µM IL-37 for 0.5 h, 100 ng/ml ox-LDL, 25 mM glucose for 24 h. (A and B) Western blot analysis was used to analyze the protein level of GPX4. (C and D) C11 BODIPY staining was used to analyze lipid peroxidation levels of macrophage cell membranes in different groups. (E) MDA level in different group. (F) GSH level in different group. (G) SOD level in different group. *P<0.05 vs. control group, ***P<0.001 vs. control group and #P<0.05 vs. HG/ox-LDL, ###P<0.001 vs. HG/ox-LDL. Each dot represents a biological repetition. HG, high glucose; GPX4, glutathione peroxidase 4; MDA, malondialdehyde; GSH, reduced glutathione; SOD, superoxide dismutase.