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. 2023 May 3;25(6):289. doi: 10.3892/etm.2023.11988

Figure 3.

Figure 3

IL-37 enhances NRF2 nuclear translocation. The macrophages were divided into three groups: i) CTRL group; ii) HG/ox-LDL group: The macrophages were treated with 100 ng/ml ox-LDL and 25 mM glucose for 24 h; and iii) IL-37 group: 30 µM IL-37 for 0.5 h, 100 ng/ml ox-LDL, 25 mM glucose for 24 h. (A and B) Immunofluorescence was used to observe the level of NRF2 in the nuclei of different groups. (C and D) Macrophage nuclear proteins were extracted and western blotting was used to evaluate the level of NRF2 in the nucleus. (E and F) Macrophage total and cytoplasmic proteins were extracted and western blotting was used to evaluate the level of NRF2 in the nucleus. *P<0.05, ***P<0.001 vs. control group and #P<0.01, ###P<0.001 vs. HG/ox-LDL. Each dot represents a biological repetition. NRF2, nuclear factor erythroid 2-related factor 2; HG, high glucose.