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. 2023 May 3;25(6):289. doi: 10.3892/etm.2023.11988

Figure 4.

Figure 4

ML385 abrogates the ferroptosis protection of IL-37. The macrophages were divided into four groups: i) CTRL group; ii) HG/ox-LDL group: The macrophages were treated with 100 ng/ml ox-LDL and 25 mM glucose for 24 h; iii) IL-37 group: 30 µM IL-37 for 0.5 h, 100 ng/ml ox-LDL, 25 mM glucose for 24 h; and iv) ML385 group: 5 µM ML385 for 0.5 h, 30 µM IL-37 for 0.5 h, 100 ng/ml ox-LDL, 25 mM glucose for 24 h. (A) Cell Counting Kit-8 assay was used to evaluate the cell viability in different groups. (B) LDH assay was used to analyze the LDH content in cell supernatant. (C and D) Western blotting was used to detected the level of GPX4 in different groups. (E and F) C11 BODIPY staining was used to analyze lipid peroxidation levels of macrophage cell membranes in different groups. ***P<0.001 vs. control group, ###P<0.001 vs. HG/ox-LDL and $$$P<0.001 vs. IL-37/HG/ox-LDL. Each dot represents a biological repetition. HG, high glucose; LDH, lactate dehydrogenase; GPX4, glutathione peroxidase 4.