Table 2. Read alignment error profiles for E. coli tRNAfMet, tRNALys, and tRNAPhea.
| synthetic |
biological |
|||||
|---|---|---|---|---|---|---|
| tRNAfMet | tRNALys | tRNAPhe | tRNAfMet | tRNALys | tRNAPhe | |
| median alignment identity | 86.5% | 86.3% | 84.0% | 78.9% | 75.3% | 76.1% |
| median read coverage | 98.3% | 98.4% | 97.3% | 96.2% | 97.2% | 95.7% |
| median mismatches per aligned base | 5.7% | 5.8% | 7.7% | 12.9% | 15.5% | 15.2% |
| median deletions per read base | 4.1% | 4.1% | 4.4% | 4.0% | 4.3% | 4.1% |
| median insertions per read base | 1.4% | 1.5% | 1.8% | 2.9% | 1.7% | 3.2% |
a
Alignments were generated using references which did not contain adapter sequences and were filtered for MAPQ > 0. The first column to the left indicates the relevant error profile metrics. The following three columns contain the error profiles for synthetic E. coli tRNAfMet, tRNALys, and tRNAPhe. The last three columns contain the error profiles for biological E. coli tRNAfMet, tRNALys, and tRNAPhe.