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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: J Med Virol. 2023 Mar;95(3):e28673. doi: 10.1002/jmv.28673

Figure 1.

Figure 1.

Mouse mAb clones with high affinity to the RBD of the original Wuhan-Hu-1 strain. Mouse hybridomas were generated using the original RBD as the immunogen. Five mAb clones (17B10, 17A7, 2G5, 3A6 and 20B5) were selected and evaluated for RBD binding by bio-layer interferometry (BLI). (A) Representative BLI sensorgrams of individual mAbs binding to immobilized RBD. All five mAbs were 3-fold diluted from the initial concentration of 15 μg/mL and collected data were applied to a built-in 1:2 global curve-fitting model with Kd values shown. (B) Pseudovirus-based neutralization against SARS-CoV-2 spike (D614).