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Effect of FIS on growth rate-dependent regulation of rrnB P1 transcription in wild-type (A and D), rpoCΔ215–220 (B and E), and rpoBRH454 (C and F) strains. (A to C) Transcription from the rrnB P1 promoter without FIS sites (−61 to +50). (D to F) Transcription from the rrnB P1 promoter containing three FIS sites (−154 to +50). Promoter activities were determined from β-galactosidase activities of promoter-lacZ fusions. Growth rates of cultures were varied as described previously (7).
